《植物生理学报》 2009, 45(4): 345-350

大豆重组自交系群体(ZKS-HX)遗传图谱的构建和形态标记的定位

吕祝章^1,*, 丁立孝¹, 田纪春², 常汝镇³, 邱丽娟³

1 日照职业技术学院, 山东日照 276826; 2 山东农业大学农学院, 山东泰安 271018; 3 中国农业科学院作物科学研究所, 国家农 作物基因资源与遗传改良重大科学工程, 农业部作物种质资源与生物技术重点开放实验室, 北京 100081

通信作者：吕祝章；E-mail: ytzhuzhang@tom.com；Tel: 0633-8172898

摘　要：

以大豆品种 ‘ 合丰 25’ 为母本, 半野生大豆 ‘ 新民 6 号 ’ 为父本杂交得到的 F代 122 个重组自交系为试验材料, 构建了含有 124 个 SSR 标记、1 个 EST 标记、3 个形态学标记的大豆遗传图谱。此图谱覆盖的基因组长度为 2 348.3 cM, 标记 间平均距离为 18.3 cM。每个连锁群长度范围为 15.1~195.9 cM 之间, 标记数范围 2~10 个。本文将控制茸毛色(Pb)基因定 位于 LG06-C2 连锁群上, 与 Sat_402 的遗传距离为 39.6 cM; 控制叶耳色(Le)、花色(W)基因定位于 LG12-F 连锁群上, 它们 之间的遗传距离为 9.9 cM, 与两边的 Satt348、Sat_240 标记遗传距离分别为 13.3 cM 和 10.5 cM。

关键词：大豆; 重组自交系; 遗传图谱; SSR; EST

收稿：2008-10-20   修定：2008-12-22

资助：国家自然科学基金(30490250)。

A Genetic Map of Soybean Using a Recombinant Inbred Line Population (ZKSHX) and Mapping of Morphological Markers

LÜ Zhu-Zhang^1,*, DING Li-Xiao¹, TIAN Ji-Chun², CHANG Ru-Zhen³, QIU Li-Juan³

¹Rizhao Polytechnic College, Rizhao, Shandong 276826, China; ²College of Agronomy, Shandong Agricultural University, Taian, Shandong 271018, China; ³Institute of Crop Sciences, National Key Facility of Crop Gene Resources and Genetic Improvement, Key Laboratory of Crop Germplasm & Biotechnology (MOA), Chinese Academy of Agricultural Sciences, Beijing 100081, China

Corresponding author: LÜ Zhu-Zhang; E-mail: ytzhuzhang@tom.com; Tel: 0633-8172898

Abstract:

A F2:9 RIL (recombinant inbred line) population with 122 lines was derived from a cross of ‘Hefeng 25’ as a female parent and ‘Xinmin 6’ as a male parent. A new soybean molecular genetic map was constructed by using Mapmaker. The new soybean genetic map covered 2 348.3 cM, and the average distance among markers was 18.3 cM. The length of linkage group varied from 15.1 cM to 195.9 cM, and the markers on each linkage group varied from 2 to 10. 124 SSRs, 1 EST and 3 morphology markers were located in this map. A pubescence color (Pb) gene was located on LG06-C2 linkage group, 39.6 cM far from the marker of Sat_402. The auricle color gene (Le) and flower color gene (W) were located on LG12-F linkage group, the distance between Le and W was 9.9 cM, the distances between the two genes and the markers of Satt348 and Sat_240 were 13.3 cM and 10.5 cM, respectively.

Key words: soybean; RIL; genetic map; SSR; EST